Action number: CA21135
Grantee name: Tina Kolenc Milavec
Dissemination: During my 2-months long stay at the University Medical Center in Amsterdam, in the group of Febe van Maldegem, PhD, I learnt all about the Imaging mass cytometry (IMC). This novel technique enables us to determine spatial characteristics of a tissue and observe interactions between cells. I was using IMC to determine changes in the interactions within the glioblastoma microenvironment, which arise from applying treatment (radiotherapy and/or chemotherapy) to glioblastoma organoids. Understanding how cell interactions change after therapy will hopefully help us to unveil the mechanisms underlying therapy resistance.
![](https://www.immuno-model.eu/wp-content/uploads/2024/05/image002-e1716456281966-1024x989.jpg)
Tina Kolenc Milavec (left) and PhD student Sofie Koomen (right) trying to prepare organoids from the lung cancer tissue we obtained from a patient after surgery.
![](https://www.immuno-model.eu/wp-content/uploads/2024/05/image004-1024x768.jpg)
Me (Tina) in the laboratory, doing staining of FPPA-fixed glioblastoma tissue slices with the antibody mix for IMC analysis.
![](https://www.immuno-model.eu/wp-content/uploads/2024/05/image006-1024x766.jpg)
Me (left) and Sofie Koomen (right) at the Spring conference of the Dutch Society for immunology (NVVI) in Tiel, Netherlands. I sure learnt a lot of new things about immunology.
Short news/reports about the STSM were also posted on Instagram and LinkedIn.